Strain Data Sheet
RBRC12395
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC12395 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6J-Gt(ROSA)26Sor<em1(CAG-HtrA1/Myc)Tiwata> |
| Former Common name | Rosa-CAG-HtrA1 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | C-terminally Myc-tagged mouse HtrA1 under the CAG promoter was inserted into the mouse Rosa locus by a modified CRISPR/Cas9 system. Injection and transplantation were performed by Institute of Immunology (Tochigi, Japan). The line is maintained by crossing the KI-hetero male to C57BL/6 female. Viable in heterozygous and homozygous. Expression of HtrA1-Myc is detected by western blotting for HtrA1 and Myc. |
| Strain description | This mouse was generated to stabely express C-terminally Myc-tagged mouse HtrA1 under the CAG promoter. |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<em1(CAG-HtrA1/Myc)Tiwata> | endonuclease-mediated mutation 1, Takeshi Iwata | |||
| HtrA1 | HtrA serine peptidase 1 | 6 | HtrA1 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| human Myc tag | 6 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Mouse HtrA1 cDNA, human cytomegalovirus hCMV immediate early enhancer, Chicken beta-actin promoter, Chicken/rabbit chimera between introns from chicken beta-actin and rabbit beta-globin, human Myc tag |
| Research application | |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Takeshi Iwata (National Hospital Organization Tokyo Medical Center) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |