Strain Data Sheet

RBRC12185

Strain Information

Image
BRC No.RBRC12185
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Aif1-tTA)75Kftnk Bdnf<tm1.1(tetO)Kftnk>
Former Common nameIba1-tTA/BDNF tetO knockin double transgenic
H-2 Haplotype
ES Cell lineG0G1
Background strain
Appearance
Strain development寄託者が作出。G0G1 (阪大微研の岡部勝先生が樹立, 129 と B6 の hybrid) で作成。G0G1 (阪大微研の岡部勝先生が樹立, 129 と B6 の hybrid) で作成。C57BL/6J（日本クレア）に 5 代以上 backcross してある
Strain descriptiontetO が、BDNF gene の exon 4 にノックインされている（Tanaka KF et al., Biol Psychiatry 2010 の方法に従う）。Iba1-tTA マウスとの組み合わせでミクログリアで Isoform 4 の発現が増加する（Komori et al., Mol Psychiatry 2024）。
Colony maintenance
ReferencesMolecular Psychiatry. in press 38243072

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bdnfbrain derived neurotrophic factor2Bdnf<tm1.1(tetO)Kftnk>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tetOtetracycline operator (E. coli)2tetO

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA

Ordering Information

供与核酸mouse Iba1 promoter, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, Bovine Growth Hormone polyA additional signal, yeast FRT (flippase recombination target) site, E. coli Tetracycline operator site, サイトメガロウイルス CMV 最小プロモーター、phage P1 loxP sites, mouse Bdnf genomic DNA
Research applicationTet system
提供条件条件を付加しない。
Depositor田中　謙二（慶應義塾大学）
Strain Status生体のアイコン生体
Strain Availability受注後、検査を施して提供
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data