Strain Data Sheet

RBRC12146

Strain Information

Image
BRC No.RBRC12146
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6.Cg-Actb<em2(tetO-FLEX-hM3Dq/mCherry)Kta>
Former Common nameActb-TRE-FLEX-hM3Dq
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesBMC Genomics, 17(1):979 (2016). 27894274
Genome Biology, 16: 87 (2015). 25924609

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)5mCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox2272phage P1 loxP variantlox2272

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPloxP

Ordering Information

Donor DNAマウス Actb, 大腸菌 tetO, サイトメガロウイルス CMV minimal promoter, ウサギ Beta globin intron, バクテリオファージ loxP, lox2272, human CHRM3, GH polyA, Discosoma sp. mCherry, Woodchuck hepatitis virus WPRE, Streptococcus pyogenes crRNA、tracrRNA（gRNA）
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genome Biology, 16: 87 (2015).
DepositorKohichi Tanaka (Tokyo Medical and Dental University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (TBUSAMTA_Nonprofit_BROAD)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data