Strain Data Sheet

RBRC12145

Strain Information

Image
BRC No.RBRC12145
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6.Cg-Actb<em1(tetO-FLEX-EGFP)Kta>
Former Common nameActb-TRE-FLEX-EGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice.
Tomomi Aida, Keiho Chiyo, Takako Usami, Harumi Ishikubo, Risa Imahashi, Yusaku Wada, Kenji F Tanaka, Tetsushi Sakuma, Takashi Yamamoto, Kohichi Tanaka
Genome Biology, 16: 87 (2015). 25924609

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actb
MGI:87904		actin, beta5Actb<em1(tetO-FLEX-EGFP)Kta> endonuclease-mediated mutation 1, Kohichi Tanaka
  • autosomal dominant nonsyndromic hearing loss 20(MedGEN)

  • Baraitser-Winter syndrome 1(MedGEN)

  • Baraitser-winter syndrome 2(MedGEN)
    • more 1 Diseases
  • developmental malformations-deafness-dystonia syndrome(MedGEN)
    • EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)5EGFP
    GH Growth hormone polyA (human)GH
    lox2272 phage P1 loxP variantlox2272
    loxP phage P1 loxPloxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAE. coli tetO (tetracycline operator), Cytomegalovirus CMV minimal promoter, rabbit Beta globin intron, Phage P1 loxP site, lox2272 site, jellyfish EGFP cDNA, human GH polyA, mouse Actb gene, Streptococcus pyogenes crRNA, tracrRNA (sgRNA), Cas9 (mRNA)
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genome Biology, 16: 87 (2015).
    DepositorKohichi Tanaka (Tokyo Medical and Dental University)
    Strain Statusan icon for Live miceLive mice
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data