Strain Data Sheet

RBRC12122

Strain Information

Image
BRC No.RBRC12122
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Iqgap3<tm2.1(cre/ERT2)Itoy>
Former Common nameIqgap3-CreERT2 or Iqgap3-2A-CreERT2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development1. The Iqgap3 gene (NCBI Reference Sequence: NM_001033484.1) is located on mouse chromosome 3.Thirty-eight exons have been identified, with the ATG start codon in exon 1 and the TGA stop codon in exon 38.2. In the targeting vector, the TGA stop codon will be replaced with the “2A-CreERT2” cassette.3. To engineer the targeting vector, homology arms will be generated by PCR using BAC clone from the C57BL/6 library as template.4. In the targeting vector, the Neor cassette will be flanked by SDA (self-deletion anchor) sites. DTA will be used for negative selection.5. C57BL/6 ES cells will be used for gene targeting.
Strain descriptionIqgap3 is a cytosolic scaffold protein mainly expressed in stem/progenitor cells. Iqgap3-CreERT2 mouse strain can induce or deplete genes of interest in Iqgap3+ stem/progenitor cells due to the Cre/LoxP system.Endogenous Iqgap3 expression is intact. The mice do not show abnormality, and the female mice can get pregnant normally.
Colony maintenance1. Targeted C57BL/6 ES cell clone was injected into C57BL/6 albino embryos, which were then re-implanted into CD-1 pseudo-pregnant females.2. Founder animals were identified by their coat color, their germline transmission was confirmed by breeding with C57BL/6 females and subsequent genotyping of the offspring.3. Heterozygous targeted mice were generated and maintain strain by crossing with wild type C57BL/6 mice.
ReferencesGut, 70(10)1833-1846 (2021). 33293280

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iqgap3IQ motif containing GTPase activating protein 33Iqgap3<tm2.1(cre/ERT2)Itoy>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor3ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide3P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase3cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site3rox

Ordering Information

供与核酸2A : P2A self-cleaving peptide. This sequence is derived from porcine teschovirus-1.(Tang et al., Vet Res2016.CreERT2 : Fusion protein of Cre-recombinase (Phage P1) and mutated estrogen receptor (human).SDA site (Rox: Phage D6)Tamoxifen induce translocation of CreERT2 into nucleus and remove loxP sequence
Research applicationCre/loxP system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Gut, 70(10)1833-1846 (2021).
研究成果の公表にあたって謝辞の表明を必要とする。
A recipient shall acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
Depositor伊藤　嘉明（National University of Singapore）
Strain Status凍結精子のアイコン凍結精子
Strain Availability受注後、検査を施して提供
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data