Strain Data Sheet
RBRC12122
Strain Information | |
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| Image | |
| BRC No. | RBRC12122 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Iqgap3<tm2.1(cre/ERT2)Itoy> |
| Former Common name | Iqgap3-CreERT2 or Iqgap3-2A-CreERT2 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | 1. The Iqgap3 gene (NCBI Reference Sequence: NM_001033484.1) is located on mouse chromosome 3.Thirty-eight exons have been identified, with the ATG start codon in exon 1 and the TGA stop codon in exon 38.2. In the targeting vector, the TGA stop codon will be replaced with the “2A-CreERT2” cassette.3. To engineer the targeting vector, homology arms will be generated by PCR using BAC clone from the C57BL/6 library as template.4. In the targeting vector, the Neor cassette will be flanked by SDA (self-deletion anchor) sites. DTA will be used for negative selection.5. C57BL/6 ES cells will be used for gene targeting. |
| Strain description | Iqgap3 is a cytosolic scaffold protein mainly expressed in stem/progenitor cells. Iqgap3-CreERT2 mouse strain can induce or deplete genes of interest in Iqgap3+ stem/progenitor cells due to the Cre/LoxP system.Endogenous Iqgap3 expression is intact. The mice do not show abnormality, and the female mice can get pregnant normally. |
| Colony maintenance | 1. Targeted C57BL/6 ES cell clone was injected into C57BL/6 albino embryos, which were then re-implanted into CD-1 pseudo-pregnant females.2. Founder animals were identified by their coat color, their germline transmission was confirmed by breeding with C57BL/6 females and subsequent genotyping of the offspring.3. Heterozygous targeted mice were generated and maintain strain by crossing with wild type C57BL/6 mice. |
| References | Gut, 70(10)1833-1846 (2021). 33293280 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ERT2estrogen receptor3ERT2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptide3P2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase3cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site3rox |
Ordering Information | |
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| Donor DNA | 2A : P2A self-cleaving peptide. This sequence is derived from porcine teschovirus-1.(Tang et al., Vet Res2016.CreERT2 : Fusion protein of Cre-recombinase (Phage P1) and mutated estrogen receptor (human).SDA site (Rox: Phage D6)Tamoxifen induce translocation of CreERT2 into nucleus and remove loxP sequence |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Gut, 70(10)1833-1846 (2021).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. A recipient shall acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiaki Ito (National University of Singapore) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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