Strain Data Sheet
RBRC12121
Strain Information | |
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| Image | |
| BRC No. | RBRC12121 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Iqgap3<tm1.1(tdTomato)Itoy> |
| Former Common name | Iqgap3-tdTomato or Iqgap3-2A-tdTomato |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | 1. The Iqgap3 gene (NCBI Reference Sequence: NM_001033484.1) is located on mouse chromosome 3.Thirty-eight exons have been identified, with the ATG start codon in exon 1 and the TGA stop codon in exon 38.2. In the targeting vector, the TGA stop codon will be replaced with the “2A-tdTomato” cassette.3. To engineer the targeting vector, homology arms will be generated by PCR using BAC cloneRP24-71K14 or RP23-68H24 from the C57BL/6 library as template.4. In the targeting vector, the Neor cassette will be flanked by SDA (self-deletion anchor) sites. DTA will be used for negative selection.5. C57BL/6 ES cells will be used for gene targeting.1. Targeted C57BL/6 ES cell clone was injected into C57BL/6 albino embryos, which were then re-implanted into CD-1 pseudo-pregnant females.2. Founder animals were identified by their coat color, their germline transmission was confirmed by breeding with C57BL/6 females and subsequent genotyping of the offspring.3. Heterozygous targeted mice were generated and maintain strain by crossing with wild type C57BL/6 mice. |
| Strain description | Iqgap3 is a cytosolic scaffold protein mainly expressed in stem/progenitor cells. Iqgap3-tdTomato mouse strain can be used to monitor the expression pattern of Iqgap3 by red fluorescent protein tdTomato.Endogenous Iqgap3 expression is intact. The mice do not show abnormality, and the female mice can get pregnant normally. |
| Colony maintenance | |
| References | Gut, 70(10)1833-1846 (2021). 33293280 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptide3P2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)3tdTomato |
Ordering Information | |
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| Donor DNA | 2A : P2A self-cleaving peptide. This sequence is derived from porcine teschovirus-1.(Tang et al., Vet Res 2016.tdTomato : Tandem dimer (td) Tomato. This red fluorescent protein was derived from mutant of DsRed.SDA site (Rox: Phage D6) |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Gut, 70(10)1833-1846 (2021).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. A recipient shall acquire a prior written consent from the Depositor prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiaki Ito (National University of Singapore) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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