Strain Data Sheet

RBRC12108

Strain Information

Image
BRC No.RBRC12108
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pigo<em4Ymra>
Former Common namePigo+/-
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesNat. Commun., 13, 3107 (2022). 35661110

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pigophosphatidylinositol glycan anchor biosynthesis, class O4Pigo<em4Ymra>

Ordering Information

Donor DNAhuman hU6 promoter, CMV CMV enhancer (CBh) chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Escherichia coli LacZ, T7 phage T7 promoter, Escherichia coli lac promoter, Mouse a part of Pigo gene
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 13, 3107 (2022).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Yoshiko Murakami using the Approval Form. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorTaroh Kinoshita (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data