Strain Data Sheet

RBRC11928

Strain Information

Image
BRC No.RBRC11928
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N.FVB-Tg(Thy1-GCaMP6-actin/mCherry)1ShiSatm
Former Common nameB6N.FVB-Thy1-G6-actin-mCherry-Tg #6
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesPLoS One. 7(12):e51286 (2012). 23240011

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ACTBHuman beta actinUNACTB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F2Afoot-and-mouth disease virus (FMD) 2A peptideUNF2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP6RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Ordering Information

Donor DNAMouse Thy1 promoter, G-CaMP6-actin(RSET tag, Chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP), Rat calmodulin (CaM), linker, Human beta actin), Discosoma sp. mCherry cDNA, Foot-and-Mouth Disease Virus 2A peptide (F2A), Mouse poly A, Woodchuck hepatitis virus WPRE
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 7(12):e51286. (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not—for profit institution for a not—for—profit research. The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR's permission.
DepositorMasaaki Sato (Hokkaido University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data