Strain Data Sheet

RBRC11900

Strain Information

Image
BRC No.RBRC11900
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Spic<tm1.2Ksho>
Former Common nameSpi-C KO
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005. The neomycin resistance gene was deleted and backrossed to C57BL/6 mice by Wataru Ise, Osaka University Immunology Frontier Research Center (IFReC), around 2011-2013.
Strain descriptionSpi-C is an Ets family transcription factor. In Spi-C KO mice, the Spi-C gene locus was disrupted by cre recombinase-mediated depletion of the exon-containing regions flanked by two loxP sites. Spi-C-deficient mice show various phenotypes, such as ablation of splenic red pulp macrophage and defects in intestinal macrophage functions.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 115:8418-8423 (2018). 30061415

Health Report

Examination Date / Room / Rack2024/03/11Room:3-2Rack:D
2023/12/11Room:3-2Rack:D
2023/09/11Room:3-2Rack:D
2023/06/13Room:3-2Rack:D
2023/03/13Room:3-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SpicSpi-C transcription factor (Spi-1/PU.1 related)10Spic<tm1.2Ksho>targeted mutation 1.2, Tsuneyasu Kaisho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse Spic genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 115:8418-8423 (2018).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Tsuneyasu Kaisho) and CO-DEVELOPER (Dr. Wataru Ise) using the Approval Form (form D). (Contact info: Tsuneyasu Kaisho <tkaisho@wakayama-med.ac.jp>) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data