Strain Data Sheet

RBRC11898

Strain Information

Image
BRC No.RBRC11898
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Aldoc<tm1(Venus)Ksak>/Isugi
Former Common nameAldoc<tm1Isugi>, Aldoc<Venus/Venus>, Aldoc-Venus:C7BL/6N, Alsoc-Venus knock-in mice, MGI ID: 5620954
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentThe Aldoc-Venus mouse strain was proposed by Izumi Sugihara and produced by Kenji Sakimura’s Laboratory in Niigata University in 2009-2010. The generation of the Aldoc-Venus mice was supported by a Grant-in-Aid for Scientific Research on Innovative Areas (Comprehensive Brain Science Network) from the Ministry of Education, Science, Sports and Culture of Japan. The stain can be maintained by mating between homozygotes. No apparent phenotypic features except for the venus expression in the brain.
Strain descriptionA gene of mutated green fluorescent protein (Venus) is inserted to the exon 2 of the Aldoc gene in Aldoc-Venus mice. In this mouse strain, the knocked in Venus gene is expressed under the same transcriptional control as the Aldoc gene. Consequently, zebrin (aldolase C, Aldoc)- positive Purkinje cells, which are arranged in the longitudinal striped pattenrn in the cerebellum, are visualized by Venus expression in Aldoc-Venus mice. Additionally astrocytes in the cebtral nervous system are mildly visualuzed by week Venus expression. Many retinal neurons are also visualuzed by Venus expression.
Colony maintenance
ReferencesPLoS One, 9(1):e86679 (2014). doi: 10.1371/journal.pone.0086679. eCollection 2014. 24475166

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aldocaldolase C, fructose-bisphosphate11Aldoc<tm1(Venus)Ksak>targeted mutation 1, Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)11Venus

Ordering Information

供与核酸マウス マウス由来 PGK 遺伝子 promoter, 大腸菌 大腸菌由来 Neo 耐性遺伝子, マウス マウス由来 PGK poly(A)シグナル, 出芽酵母 フリッパーゼ認識標的部位配列 FRT, オワンクラゲ オワンクラゲ Venus(EGFP の変異体)遺伝子, phage T7 gb2 promoter,
Research application
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。PLoS One. Jan 27;9(1):e86679.(2014) doi: 10.1371/journal.pone.0086679. eCollection 2014.
研究成果の公表にあたってVenusの開発者である宮脇敦史先生への謝辞の表明並びに指定する文献を引用する。Nat. Biotechnol., 20, 87-90 (2002).
Depositor杉原　泉（国立大学法人東京医科歯科大学）
Strain Status凍結胚のアイコン凍結胚
Strain Availability受注後、検査を施して提供
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data