Strain Data Sheet

RBRC11689

Information1

Image
BRC No.RBRC11689
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6;Cg-Oxtr<em4(3xHA/T2A/icre/ERT2)Yinn>
Former Common nameOxtr<3xHA-iCreERT2>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Yukiko U. Inoue and Takayoshi Inoue, National Center of Neurology and Psychiatry in 2022. This strain was generated using fertilized eggs derived from (C57BL/6 x C3H)F1. Mixed genetic background.
Strain descriptionicre/ERT2 knockin mice via 3xHA/T2A at the C-terminus of the Oxtr gene. Homozygous mice are fertile.
Colony maintenance
ReferenceseNeuro, 9(1):ENEURO.0423-21.2022 (2022). 35082173

Health Report

Examination Date / Room / Rack2023/02/27Room:4-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Oxtroxytocin receptor6Oxtr<em4(3xHA/T2A/icre/ERT2)Yinn>endonuclease-mediated mutation 4, Yukiko U Inoue

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor6ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)6T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
icrephage P1 codon improved Cre (iCre)6icre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Information2

Donor DNAhuman influenza virus HA (hemagglutinin) tag sequence, Thosea asigna virus 2A peptide (insect), SV40 nls, Phage P1 Cre (codon-improved Cre recombinase), human Estrogen receptor (ERT2)
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. eNeuro. Feb 15;9(1):ENEURO.0423-21.2022 (2022)For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakayoshi Inoue (National Institute of Neuroscience)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data