Strain Data Sheet

RBRC11656

Strain Information

Image
BRC No.RBRC11656
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ldhc<em1(LDHC)Osb>
Former Common namehLDHC-KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from C57BL/6N were used to generate this line. C57BL/6N genetic background.
Strain descriptionKnock-in mice of human LDHC cDNA at the Ldhc locus.
Colony maintenance
References
Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs.
Rie Iida-Norita, Haruhiko Miyata, Yuki Kaneda, Chihiro Emori, Taichi Noda, Tatsuya Nakagawa, Martin M Matzuk, Masahito Ikawa
Andrology, (2022). 36464740

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit bata-globin polyA7HBB2
LDHC lactate dehydrogenase C (human)7LDHC
Ldhc
MGI:96764		lactate dehydrogenase C7Ldhc<em1(LDHC)Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Human LDHC cDNA, Mouse a part of Ldhc gene, Rabbit beta-globin polyadenylation signal, Escherichia coli Lac Z,  T7 phage T7 promoter, T3 phage T3 promoter,  Escherichia coli lac promoter, Escherichia coli ampicillin resistant gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Andrology. doi: 10.1111/andr.13359 (2022).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data