Strain Information | |
---|---|
Image | |
BRC No. | RBRC11627 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | B6D2-Pabpn1l<em1Osb> Tg(Zp3-Pabpn1l/PA/1D4)1Osb |
Former Common name | Pabpn1l<-13/wt>, Zp3-Pabpn1l-PA/1D4 Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
Strain description | Compound strain in which C-terminally PA/1D4-tagged Pabpn1l is expressed under the control of Zp3 promoter and Pabpn1l gene mutation. 13 bp deletion at the Pabpn1l locus. |
Colony maintenance | |
References |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pabpn1lpoly(A)binding protein nuclear 1-like8Pabpn1l<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter 1D41D4 tag (synthetic)UN1D4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PAPA tag (GVAMPGAEDDVV) (synthetic) human PodoplaninUNPA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pabpn1lpoly(A)binding protein nuclear 1-likeUNPabpn1lmouse Zp3 promoter |
Ordering Information | |
---|---|
Donor DNA | human hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Pabpn1l gene, Mouse Zp3 promoter, Bovine Glowth hormone polyadenylation signal, Mouse Pabpn1l cDNA, Synthetic PA tag and 1D4 tag |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
---|
No Data |