Strain Data Sheet

RBRC11627

Strain Information

Image
BRC No.RBRC11627
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Pabpn1l<em1Osb> Tg(Zp3-Pabpn1l/PA/1D4)1Osb
Former Common namePabpn1l<-13/wt>, Zp3-Pabpn1l-PA/1D4 Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionCompound strain in which C-terminally PA/1D4-tagged Pabpn1l is expressed under the control of Zp3 promoter and Pabpn1l gene mutation. 13 bp deletion at the Pabpn1l locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pabpn1lpoly(A)binding protein nuclear 1-like8Pabpn1l<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
1D41D4 tag (synthetic)UN1D4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PAPA tag (GVAMPGAEDDVV) (synthetic) human PodoplaninUNPA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pabpn1lpoly(A)binding protein nuclear 1-likeUNPabpn1lmouse Zp3 promoter

Ordering Information

Donor DNAhuman hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Pabpn1l gene, Mouse Zp3 promoter, Bovine Glowth hormone polyadenylation signal, Mouse Pabpn1l cDNA, Synthetic PA tag and 1D4 tag
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data