Strain Data Sheet
RBRC11619
Strain Information | |
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| Image | |
| BRC No. | RBRC11619 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Txn1<tm1Ksak> |
| Former Common name | Thioredoxin(Trx) flox (Neo) mouse |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Kenji Sakimura, Niigata University in 2022. Mice were generated using RENKA ES cells derived from C57BL/6N. C57BL/6N genetic background. |
| Strain description | Txn1 flox(Neo) mice. Frt-flanked Neo cassette with a loxP at 5’ is inserted at intron 1. Another loxP is inserted at intron 2. |
| Colony maintenance | |
| References | Recruitment of thioredoxin-like domains into prostaglandin synthases. Hiromi Daiyasu, Kikuko Watanabe, Hiroyuki Toh Biochem. Biophys. Res. Commun., 369(2):281-6.(2008). 18307977Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily. Moriuchi H, Koda N, Okuda-Ashitaka E, Daiyasu H, Ogasawara K, Toh H, Ito S, Woodward D F, Watanabe K J. Biol. Chem., 283, 792-801 (2008). 18006499 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 4 | Frt | ||||
| Txn1 MGI:98874 | thioredoxin 1 | 4 | Txn1<tm1Ksak> | targeted mutation 1, Kenji Sakimura | |||
| loxP | phage P1 loxP | 4 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 4 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | P1ファージ（クラス1） 病原性なし P1 ファージ部位特異的組換え認識配列 loxP (M10287), 大腸菌 病原性なし ネオマイシン耐性遺伝子, マウス 病原性なし pgk プロモーター, マウス 病原性なし pgk-PolyA, 酵母 病原性なし FRT配列 |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Biol Chem. 2008 Jan 11;283(2):792-801. Biochem Biophys Res Commun. 2008 May 2;369(2):281-6.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Kenji Sakimura (Niigata University) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |