Strain Data Sheet

RBRC11617

Strain Information

Image
BRC No.RBRC11617
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Prxl2b<tm1Ksak>
Former Common nameProstamide/prostaglandin F synthase (PM/PGFS) flox (Neo) mouse
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Kenji Sakimura, Niigata University in 2022. Mice were generated using RENKA ES cells derived from C57BL/6N. C57BL/6N genetic background.
Strain descriptionPrxl2b flox(Neo) mice. A loxP is inserted at intron 1 and Frt-flanked Neo cassette with another loxP at 3’ is inserted at intron 3.
Colony maintenance
ReferencesProstaglandins Leukot Essent Fatty Acids. Feb;165:102235.(2021) 33418484
J. Biol. Chem., 283, 792-801 (2008). 18006499

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prxl2bperoxiredoxin like 2B4Prxl2b<tm1Ksak>targeted mutation 1, Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAP1ファージ（クラス1） 病原性なし P1ファージ部位特異的組換え認識配列 loxP (M10287), 大腸菌　病原性なし ネオマイシン耐性遺伝子, マウス 病原性なし pgk プロモーター, マウス 病原性なし pgk-PolyA, 酵母 病原性なし FRT配列
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Biol Chem. 2008 Jan 11;283(2):792-801. Prostaglandins Leukot Essent Fatty Acids. 2021 Feb;165:102235.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKenji Sakimura (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data