Strain Information | |
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Image | |
BRC No. | RBRC11596 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | B6;129Ola-Ddc<em1(hKO1)Nkn> |
Former Common name | Ddc-hKO1 |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Shinpei Yamaguchi and Sheng Kit-Yeng, and deposited by Toru Nakano, Osaka University in 2022.G4 ES cells derived from 129 and B6N were used to generate this strain. Mixed genetic background. |
Strain description | hKO1 knockin mice at the stop codon of the Ddc gene via P2a peptide. |
Colony maintenance | |
References | https://doi.org/10.21203/rs.3.rs-296465/v1 |
Health Report | |
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Examination Date / Room / Rack | 2023/05/08Room:2-検Rack:C 2023/02/06Room:2-検Rack:C |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BsdAspergillus terreus blasticidin resistance gene (Bsd)11Bsdmouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptide11P2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter hKO1Humanized Kusabira-Orange (Verrillofungia concinna)11hKO1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP |
Ordering Information | |
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Donor DNA | porcine teschovirus-1 2A peptide, Humanized Kusabira-Orange (Verrillofungia concinna)1 cDNA, phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), Aspergillus terreus blasticidin resistance gene (Bsd), mouse Ddc genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Toru Nakano (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |