Strain Data Sheet

RBRC11575

Strain Information

Image
BRC No.RBRC11575
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto>
Former Common nameG-CaMP9a/R-CaMP2 KI mice
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain development開発者情報：坂本雅行、井上昌俊、尾藤晴彦開発年：2015年入手経路：新潟大学脳研究所に作製を依頼、ノックインマウスを樹立。ヘテロマウスおよびホモマウスも外見上特に異常は認められない。
Strain descriptionRosa26遺伝子座にG-CaMP9aをノックインしたレポーターマウス。組織特異的Flp発現マウスとの交配によりG-CaMP9aを、組織特異的Cre発現マウスあるいは組織特異的tTA/rtTA発現マウスとの交配によりR-CaMP2をそれぞれ発現する。In vivoカルシウムイメージングが可能となる（ただしR-CaMP2は蛍光が暗くてイメージングには使用できない）。
Colony maintenance
ReferencesCell Rep., Methods 2, 100168 (2022). 35474964
STAR Protoc., 3(2):101421 (2022). 35693213

Health Report

Examination Date / Room / Rack2024/02/26Room:4-CRack:B
2023/11/27Room:4-CRack:B
2023/08/28Room:4-CRack:B
2023/05/29Room:4-CRack:B
2023/02/27Room:4-CRack:B
2022/11/29Room:4-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto>targeted mutation 1, Haruhiko Bito

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP9aRSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA6GCaMP9aCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (human)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
His3His3 (S. cerevisiae)6His3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RCaMP2His-tag, T7-tag, Xpress-tag, ckkap, cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA6RCaMP2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TRE3GTet-On 3G (transactivator gene)6TRE3G

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site6rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site6rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

供与核酸attB1, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites,yeast FRT (flippase recombination target) sites, 2 x pA, Stop (SV40 polyA), G-CaMP9a(RSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), rabbit beta globin polyA signal, E.coli TRE3G(Tet-On 3G, transactivator gene), R-CaMP2 (His-tag, T7-tag, Xpress-tag, Ca2+/CaM-binding peptide derived from rat CaMKKα Val438–Phe463 (ckkap), Discosoma sp. cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA), human GH polyA, Phage D6 rox sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli neomycin resistance gene, synthetic polyA, attB2, yeast His3, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。(will be announced.)
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor尾藤　晴彦（東京大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability受注後、検査を施して提供
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data