Strain Information | |
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Image | |
BRC No. | RBRC11557 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S-Ostn<tm1Ncvc> |
Former Common name | Osteocrin(Ostn)LacZ/LacZ or Ostn knockout mouse, B6.129S-Ostn<tm1a(KOMP)Nmoc> |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Deposited and developed by Naoki Mochizuki and Haruko Takano, National Cerebral and Cardiovascular Center Research Institute. This strain was generated using R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> and backcrossed to C57BL/6J for 9 generations. C57BL/6J congenic line. |
Strain description | LacZ knockin mice at the Ostn locus. |
Colony maintenance | |
References | Cell Rep., 36(2):109380 (2021). 34260913 |
Health Report | |
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Examination Date / Room / Rack | 2024/03/11Room:3-6Rack:A 2023/12/11Room:3-6Rack:A 2023/09/11Room:3-6Rack:A 2023/07/18Room:3-6Rack:A 2023/06/13Room:3-6Rack:A 2023/03/13Room:3-6Rack:A 2022/12/12Room:3-6Rack:A 2022/09/12Room:3-6Rack:A |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ostnosteocrin16Ostn<tm1Ncvc>targeted mutation 1, National Cerebral and Cardiovascular Centertm1a(KOMP)Nmoc Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site16Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site16Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA16 |
Ordering Information | |
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Donor DNA | yeast FRT (flippase recombination target) sites, attB1, Mouse En2 intron (Mouse En2 intron Splicing acceptor), Ires (internal ribosomal entry site (EMCV)), E. coli beta-galactosidase, Simian Virus 40 PolyA signal, Bacteriophage P1 LoxP sites, E. coli Neomycin resistant gene, attB2, mouse Ostn genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and KOMP Repository as the source of the original allele and mice are requested. |
Depositor | Naoki MOCHIZUKI (National Cerebral and Cardiovascular Center) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |