Strain Data Sheet

RBRC11557

Strain Information

Image
BRC No.RBRC11557
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Ostn<tm1Ncvc>
Former Common nameOsteocrin(Ostn)LacZ/LacZ or Ostn knockout mouse, B6.129S-Ostn<tm1a(KOMP)Nmoc>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeposited and developed by Naoki Mochizuki and Haruko Takano, National Cerebral and Cardiovascular Center Research Institute. This strain was generated using R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> and backcrossed to C57BL/6J for 9 generations. C57BL/6J congenic line.
Strain descriptionLacZ knockin mice at the Ostn locus.
Colony maintenance
ReferencesCell Rep., 36(2):109380 (2021). 34260913

Health Report

Examination Date / Room / Rack2024/03/11Room:3-6Rack:A
2023/12/11Room:3-6Rack:A
2023/09/11Room:3-6Rack:A
2023/07/18Room:3-6Rack:A
2023/06/13Room:3-6Rack:A
2023/03/13Room:3-6Rack:A
2022/12/12Room:3-6Rack:A
2022/09/12Room:3-6Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ostnosteocrin16Ostn<tm1Ncvc>targeted mutation 1, National Cerebral and Cardiovascular Centertm1a(KOMP)Nmoc

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA16

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA16

Ordering Information

Donor DNAyeast FRT (flippase recombination target) sites, attB1, Mouse En2 intron (Mouse En2 intron Splicing acceptor), Ires (internal ribosomal entry site (EMCV)), E. coli beta-galactosidase, Simian Virus 40 PolyA signal, Bacteriophage P1 LoxP sites, E. coli Neomycin resistant gene, attB2, mouse Ostn genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and KOMP Repository as the source of the original allele and mice are requested.
DepositorNaoki MOCHIZUKI (National Cerebral and Cardiovascular Center)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data