Strain Data Sheet

RBRC11555

Strain Information

Image
BRC No.RBRC11555
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Hcrt<tm1.1(FLP)Ksak>
Former Common nameOrexin-Flippase
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDepsoted by Akihiro Yamanaka, Nagoya University in 2021. This strain was generated RENKA ES cells derived from C57BL/6N.
Strain descriptionEGFP/2A/FLP knockin mice at the translation initiation codon of the Hcrt gene.
Colony maintenanceHeterozygote x Wild-type [or crossing to C57BL/6JJcl]
References
Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice.
Chowdhury S, Hung C J, Izawa S, Inutsuka A, Kawamura M, Kawashima T, Bito H, Imayoshi I, Abe M, Sakimura K, Yamanaka A
Elife, 8:e44927 (2019). 31159922

Health Report

Examination Date / Room / Rack2024/05/27Room:4-CRack:F
2024/02/26Room:4-CRack:F
2023/11/27Room:4-CRack:F
2023/08/28Room:4-CRack:F
2023/05/29Room:4-CRack:F
2023/02/27Room:4-CRack:F
2022/11/29Room:4-CRack:F

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)11EGFP
  • narcolepsy 1(MedGEN)
    • FLPeyeast flippase recombinase11FLPe
  • narcolepsy 1(MedGEN)
    • GHgrowth hormone polyA (human)11GH
  • narcolepsy 1(MedGEN)
    • Hcrthypocretin11Hcrttargeted mutation 1.1, Kenji Sakimura
  • narcolepsy 1(MedGEN)
    • T2AThosea asigna virus 2A peptide (insect)11T2A
  • narcolepsy 1(MedGEN)
    • nlsSV40 large T antigen nuclear localization signal (NLS)11nls
  • narcolepsy 1(MedGEN)
    • roxBacteriophage D6 rox site11rox
  • narcolepsy 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAAequorea victoria EGFP cDNA, Thosea asigna virus 2A peptide (insect), SV40 nls, Saccharomyces cerevisiae Flpe recombinase, Bacteriophage D6 rox site, human GH polyA signal, mouse Hcrt genomic DNA
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, Recipient shall inform the DEPOSITOR in advance, and shall discuss and agree on the handing thereof. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of BIOLOGICAL RESOURCE, and shall obtain a prior written consent from the DEPOSITOR.
    DepositorAkihiro Yamanaka (Nagoya University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data