Strain Data Sheet

RBRC11527

Strain Information

Image
BRC No.RBRC11527
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-cat,-Gdf10)1Ncvcb
Former Common nameBMP-3b Tg (Bone morphogenetic protein-3b (BMP-3b) transgenic mice in which expression of BMP-3b was tissue specific and Cre-inducible)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Jun Hino, National Cerebral and Cardiovascular Center Hospital in 2021. C57BL/6J genetic background.
Strain descriptionTransgenic mice expressing growth differentiation factor 10 (Bmp3b) under the control of CAG promoter. and CAG and Gdf10 are separated by a floxed cat.
Colony maintenance
ReferencesInt. J. Obes. (Lond)., 41(4):483-488 (2017). 28104917

Health Report

Examination Date / Room / Rack2024/03/11Room:3-2Rack:A
2023/12/11Room:3-2Rack:A
2023/09/11Room:3-2Rack:A
2023/06/26Room:3-2Rack:A
2023/06/13Room:3-2Rack:A
2023/03/13Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gdf10growth differentiation factor 10UNGdf10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, E. coli chloramphenicol acetyltransferase, mouse BMP-3b cDNA, SV40 polyA
Research applicationCre/loxP system
Hematological Research
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. J. Obes. (Lond)., 41(4):483-488 (2017).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the Co-Developer (Ensyo Takuya) using the Approval Form.
DepositorMikiya Miyazato (National Cerebral and Cardiovascular Center)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data