Strain Data Sheet



BRC No.RBRC11518
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-App<tm2(NL-F)Tcs> Psen1<em1(P117L)Tcs>
Former Common nameApp<NL-F>Psen1<P117L> mice
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeposited by Takaomi C. Saido, RIKEN Center for Brain Science in 2022.
Strain descriptionApp knock-in mice having Swedish/Iberian (NL-F) mutations. This mutant also has a point mutation (P117L) in the Psen1 gene. Homozygous mutant mice are viable and fertile.
Colony maintenance
ReferencesNat. Neurosci., 17(5):661-663 (2014). 24728269
Nat. Commun., 9(1):2892.(2018) doi: 10.1038/s41467-018-05262-w. 30042426
J. Biol. Chem., 297(3):101004 (2021). 34329683

Health Report

Examination Date / Room / Rack2023/02/27Room:4-BRack:H


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Psen1presenilin 112Psen1<em1(P117L)Tcs>endonuclease-mediated mutation 1, Takaomi C Saido

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Appamyloid beta (A4) precursor protein16App<tm2(NL-F)Tcs>targeted mutation 2, Takaomi C. Saido

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site16Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP


Donor DNAphage P1 loxP site, yeast FRT (flipase recombination target) site, mouse App (amyloid beta (A4) precursor protein) genomic DNA (mutant type)
Research applicationCre/loxP system
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsThe BIOLOGICAL RESOURCE is the property of the RIKEN (The Institute of Physical and Chemical Research). RECIPIENT agrees to acknowledge Hiroki SASAGURI, Takashi SAITO and Takaomi C. SAIDO in an acknowledgement section. RECIPIENT also agrees to cite Nature Neuroscience 17, 661-663 (2014), J. Biol. Chem., 297(3):101004 (2021) and Nature Communications 9, 2892 (2018) in all publications obtained using BIOLOGICAL RESOURCE. RECIPIENT agrees to acknowledge DEPOSITOR in all oral presentations and written publications as related to the BIOLOGICAL RESOURCE. RECIPIENT shall keep confidential any or all information marked CONFIDENTIAL that is received from DEPOSITOR and related to the BIOLOGICAL RESOURCE including oral discussions if they are reduced to writing within thirty (30) days and are marked "CONFIDENTIAL". For-profit organizations must contact the DEPOSITOR ( for a licensing contract. If any invention is conceived and reduced to practice by RECIPIENT in the performance of the Research Plan involving the BIOLOGICAL RESOURCE during the term of this MTA, RECIPIENT agrees to promptly inform the invention to DEPOSITOR and the parties will consult each other to determine inventorship and ownership of the invention based on the respective parties contribution to the invention, before filing an application for a patent. In the case of a jointly owned application, the parties will separately enter into a joint application agreement including the sharing of ownership, patent costs and licensing income, as well as the responsible party for the application procedures etc.
DepositorTakaomi C. Saido (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data