Strain Data Sheet
RBRC11518
Information1 | |
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| Image | |
| BRC No. | RBRC11518 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | C57BL/6-App<tm2(NL-F)Tcs> Psen1<em1(P117L)Tcs> |
| Former Common name | App<NL-F>Psen1<P117L> mice |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Takaomi C. Saido, RIKEN Center for Brain Science in 2022. |
| Strain description | App knock-in mice having Swedish/Iberian (NL-F) mutations. This mutant also has a point mutation (P117L) in the Psen1 gene. Homozygous mutant mice are viable and fertile. |
| Colony maintenance | |
| References | Nat. Neurosci., 17(5):661-663 (2014). 24728269 Nat. Commun., 9(1):2892.(2018) doi: 10.1038/s41467-018-05262-w. 30042426 J. Biol. Chem., 297(3):101004 (2021). 34329683 |
Health Report | |
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| Examination Date / Room / Rack | 2023/02/27Room:4-BRack:H 2023/02/20Room:4-BRack:H |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site16Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP |
Information2 | |
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| Donor DNA | phage P1 loxP site, yeast FRT (flipase recombination target) site, mouse App (amyloid beta (A4) precursor protein) genomic DNA (mutant type) |
| Research application | Cre/loxP system FLP/frt system Mouse Models for Human Disease |
| Specific Term and Conditions | The BIOLOGICAL RESOURCE is the property of the RIKEN (The Institute of Physical and Chemical Research). RECIPIENT agrees to acknowledge Hiroki SASAGURI, Takashi SAITO and Takaomi C. SAIDO in an acknowledgement section. RECIPIENT also agrees to cite Nature Neuroscience 17, 661-663 (2014), J. Biol. Chem., 297(3):101004 (2021) and Nature Communications 9, 2892 (2018) in all publications obtained using BIOLOGICAL RESOURCE. RECIPIENT agrees to acknowledge DEPOSITOR in all oral presentations and written publications as related to the BIOLOGICAL RESOURCE. RECIPIENT shall keep confidential any or all information marked CONFIDENTIAL that is received from DEPOSITOR and related to the BIOLOGICAL RESOURCE including oral discussions if they are reduced to writing within thirty (30) days and are marked "CONFIDENTIAL". For-profit organizations must contact the DEPOSITOR (takaomi.saido@riken.jp) for a licensing contract. If any invention is conceived and reduced to practice by RECIPIENT in the performance of the Research Plan involving the BIOLOGICAL RESOURCE during the term of this MTA, RECIPIENT agrees to promptly inform the invention to DEPOSITOR and the parties will consult each other to determine inventorship and ownership of the invention based on the respective parties contribution to the invention, before filing an application for a patent. In the case of a jointly owned application, the parties will separately enter into a joint application agreement including the sharing of ownership, patent costs and licensing income, as well as the responsible party for the application procedures etc. |
| Depositor | Takaomi C. Saido (RIKEN) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |