Strain Data Sheet

RBRC11512

Strain Information

Image
BRC No.RBRC11512
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Utf1<tm1Okud>
Former Common nameUTF1 knockout mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentStrain development: The targeting vector harboring neomycin resistance gene with SV40 poly-A addition signal under control of the endogenous UTF1 enhance-promoter was transferred to E14 ES cells to replace the 1st exon of UTF1 gene. The resultant UTF1 locus can produce no UTF1 protein (even the truncated form). 開発者：「鍋島陽一先生（国立精神・神経センター（現：国立精神・神経医療研究センター）；現職：神戸医療産業都市推進機構　先端医療センター老化機構研究部　部長）」作製年度：1997年分与年度：鍋島先生より1998年
Strain descriptionThe prominent phenotype of UTF1 KO homozygous mice is “in utero growth retardation”. This phenotype is due to the growth retardation of placenta, not due to the defect of the differentiation program of embryo per se. This placental growth defect is unexpected because UTF1 gene is identified as a gene whose expression is pluripotent cell specific (Okuda, A. et al. (1998) EMBO J. 17: 2019–2032). This UTF1 KO phenotype indicates that this mouse strain may be useful to analyze not only the differentiation program of the embryo but also the placental developmental program.Homozygous mutant of C57BL/6J background is embryonic or peri-natal lethal. Some of the homozygous mutant mice of F1 (B6 and ICR) genetic background are viable. This phenotypic difference suggests that the severity of phenotype may be dependent on the genetic background (see Nishimoto,M. et al. (2013) PLoS ONE 8(7): e68119). Appearance: Black [a/a B/B C/C]Colony maintenance: Heterozygote crossing to C57BL/6JJcl
Colony maintenanceBackcross C57BL/6J (Jcl) : backcross to male C57BL/6J at least three generations. Homozygous mouse shows the “in utero growth retardation” phenotype.
ReferencesEMBO J. 17: 2019-2032 (1998). 9524124
PLoS One, 8(7):e68119 (2013). doi: 10.1371/journal.pone.0068119 23874519

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Utf1undifferentiated embryonic cell transcription factor 17Utf1<tm1Okud>targeted mutation 1, Akihiko Okuda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA additional signal7

Ordering Information

供与核酸E. coli Neomycin resistance gene, SV40 PolyA addition signal, mouse Utf1 genomic DNA
Research application
提供条件条件を付加する。
研究成果の公表にあたって譲渡者の指定する文献を引用する。PLoS One, 8(7):e68119 (2013). doi: 10.1371/journal.pone.0068119
研究成果の公表にあたって謝辞の表明を必要とする。
譲渡者は、本件リソースの提供実績と利用者の利用成果等の情報を理研BRCが譲渡者へ報告することを要求できる。理研BRCはその情報を譲渡者へ送付する。但し、本譲渡においては、提供の事前承認、利用者及び利用目的の制限、所有権等に関わる条件等は付加できない。
Depositor奥田　晶彦（埼玉医科大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data