Strain Information | |
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Image | |
BRC No. | RBRC11503 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | B6D2-Spaca6<em2Osb> Tg(Clgn-Spaca6/1D4)1Osb |
Former Common name | Spaca6<-8085+4/wt>, Spaca6-1D4 Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
Strain description | Spaca6 KO mouse generated by the CRISPR/Cas9. 8,085 bp deletion 4 bp insertion in the Spaca6 gene. This strain also has a transgene expressing Spaca6 fused with 1D4 under the control of Clgn promoter. |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 117(21) 11493-11502 (2020). 32393636 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Spaca6sperm acrosome associated 617Spaca6<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter 1D41D4 tag (synthetic)UN1D4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Spaca6sperm acrosome associated 6UNSpaca6mouse Clgn promoter |
Ordering Information | |
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Donor DNA | Mouse Calmegin promoter, Rabbit Beta-globin polyadenylation signal, Mouse Spaca6 cDNA, Synthetic 1D4, Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Spaca6 gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 117(21) 11493-11502 (2020). doi: 10.1073/pnas.1922650117. Epub 2020 May 11.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |