Strain Data Sheet

RBRC11503

Strain Information

Image
BRC No.RBRC11503
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Spaca6<em2Osb> Tg(Clgn-Spaca6/1D4)1Osb
Former Common nameSpaca6<-8085+4/wt>, Spaca6-1D4 Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionSpaca6 KO mouse generated by the CRISPR/Cas9. 8,085 bp deletion 4 bp insertion in the Spaca6 gene. This strain also has a transgene expressing Spaca6 fused with 1D4 under the control of Clgn promoter.
Colony maintenance
References
Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice.
Noda T, Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk M M, Ikawa M
Proc. Natl. Acad. Sci. USA, 117(21) 11493-11502 (2020). 32393636

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spaca6
MGI:1922452		sperm acrosome associated 617Spaca6<em2Osb> endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University
1D4 1D4 tag (synthetic)UN1D4
Spaca6 sperm acrosome associated 6UNSpaca6 mouse Clgn promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAMouse Calmegin promoter, Rabbit Beta-globin polyadenylation signal, Mouse Spaca6 cDNA, Synthetic 1D4, Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Spaca6 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 117(21) 11493-11502 (2020). doi: 10.1073/pnas.1922650117. Epub 2020 May 11.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data