Strain Information | |
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Image | |
BRC No. | RBRC11502 |
Type | CRISPR/Cas9 (Transgene) |
Species | Mus musculus |
Strain name | B6D2-Tmem95<em1Osb> Tg(Clgn-Tmem95/1D4)1Osb |
Former Common name | Tmem95<-1919/wt>, Tmem95-1D4 Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
Strain description | Tmem95 knockout mouse generated by the CRISPR/Cas9. 1919 bp deletion in the Tmem95 gene. This strain also has a transgene expressing Tmem95 fused with 1D4 under the control of Clgn promoter. |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 117(21) 11493-11502 (2020). 32393636 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tmem95transmembrane protein 9511Tmem95<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter 1D41D4 tag (synthetic)UN1D4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tmem95transmembrane protein 95UNTmem95mouse Clgn promoter |
Ordering Information | |
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Donor DNA | Mouse Calmegin promoter, Rabbit Beta-globin polyadenylation signal, Mouse Tmem95 cDNA, Synthetic 1D4, Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Tmem95 gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 117(21) 11493-11502 (2020). doi: 10.1073/pnas.1922650117. Epub 2020 May 11.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |