Strain Data Sheet

RBRC11495

Strain Information

Image
BRC No.RBRC11495
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Spaca9<em1Osb>
Former Common nameSpaca9<-3974/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionSpaca9 knockout mouse generated by the CRISPR/Cas9. 3,974 bp deletion in the Spaca9 gene.
Colony maintenance
References
FAM209 associates with DPY19L2, and is required for sperm acrosome biogenesis and fertility in mice.
Julio M Castaneda, Keisuke Shimada, Yuhkoh Satouh, Zhifeng Yu, Darius J Devlin, Masahito Ikawa, Martin M Matzuk
J. Cell Sci., 134(21):jcs259206 (2021). 34471926

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spaca9
MGI:1917237		sperm acrosome associated 92Spaca9<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Spaca9 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 134(21):jcs.259206. doi: 10.1242/jcs.259206. (2021).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data