Strain Information | |
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Image | |
BRC No. | RBRC11491 |
Type | CRISPR/Cas9 (Transgene)![]() |
Species | Mus musculus |
Strain name | B6D2-Dcst1<em2Osb> Tg(Clgn-Dcst1/3xHA)1Osb |
Former Common name | Dcst1<-1/wt>;Tg (3xHA) |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
Strain description | Dcst1 mutant mouse generated by the CRISPR/Cas9. 1 bp deletion in the Dcst1 gene. This strain also has a transgene expressing Dcst1 fused with 3xHA under the control of Clgn promoter. |
Colony maintenance | |
References | Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Taichi Noda, Andreas Blaha, Yoshitaka Fujihara, Krista R Gert, Chihiro Emori, Victoria E Deneke, Seiya Oura, Karin Panser, Yonggang Lu, Sara Berent, Mayo Kodani, Luis Enrique Cabrera-Quio, Andrea Pauli, Masahito Ikawa Commun. Biol., 5(1):332 (2022). 35393517 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Dcst1 MGI:1925022 | DC-STAMP domain containing 1 | 3 | Dcst1<em2Osb> | endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University | |||
Dcst1 | DC-STAMP domain containing 1 | UN | Dcst1 | mouse Clgn promoter | |||
HA | human influenza virus HA (hemagglutinin) tag sequence | UN | HA |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Mouse Dcst1 cDNA, Mouse Calmegin promoter, synthetic HA tag, Rabbit beta-globin polyadenylation signal, human hU6 promoter, CMV CMV enhancer(CBh), chicken chicken beta-actin promoter(CBh), SV40 SV40 nuclear localization signal,Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA,Escherichia coli ampicillin resistance gene, Mousea part of Dcst1 gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Commun Biol Apr 7;5(1):332.(2022) doi: 10.1038/s42003-022-03289-w.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | ![]() |
Strain Availability | ![]() |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |