Strain Data Sheet

RBRC11489

Strain Information

Image
BRC No.RBRC11489
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Dcst2<em3Osb>
Former Common nameDcst2<wt/del>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous male mutants are infertile.
Strain descriptionDcst2 knockout mouse generated by the CRISPR/Cas9. 7,223 bp deletion and 2 bp insertion in the Dcst2 gene.
Colony maintenance
ReferencesCommun Biol., 5(1):332 (2022). doi: 10.1038/s42003-022-03289-w. 35393517

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dcst2DC-STAMP domain containing 23Dcst2<em3Osb>endonuclease-mediated mutation 3, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Dcst2 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Commun Biol Apr 7;5(1):332.(2022) doi: 10.1038/s42003-022-03289-w.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data