Strain Data Sheet
RBRC11488
Strain Information | |
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| Image | |
| BRC No. | RBRC11488 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6D2-Dcst2<em2Osb> Tg(Clgn-Dcst2/3xHA)1Osb |
| Former Common name | Dcst<2-25/wt>;Tg (3xHA) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. Homozygous Dcst2 male mutants are infertile, but this phenotype can be rescued by the transgene. |
| Strain description | Dcst2 knockout mouse generated by the CRISPR/Cas9. 25 bp deletion in the Dcst2 gene. This strain also has a transgene expressing Dcst2 fused with 3xHA-tag (C-terminus) under the control of Clgn promoter. |
| Colony maintenance | |
| References | Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Taichi Noda, Andreas Blaha, Yoshitaka Fujihara, Krista R Gert, Chihiro Emori, Victoria E Deneke, Seiya Oura, Karin Panser, Yonggang Lu, Sara Berent, Mayo Kodani, Luis Enrique Cabrera-Quio, Andrea Pauli, Masahito Ikawa Commun. Biol., 5(1):332 (2022). 35393517 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Dcst2 MGI:2685606 | DC-STAMP domain containing 2 | 3 | Dcst2<em2Osb> | endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University | |||
| Dcst2 | DC-STAMP domain containing 2 | UN | Dcst2 | mouse Clgn promoter | |||
| HA | human influenza virus HA (hemagglutinin) tag sequence | UN | HA | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Dcst2 gene, Mouse Dcst2 cDNA, Mouse Calmegin promoter, synthetic HA tag, Rabbit beta-globin polyadenylation signal |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Commun Biol Apr 7;5(1):332.(2022) doi: 10.1038/s42003-022-03289-w.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |