Strain Data Sheet

RBRC11486

Strain Information

Image
BRC No.RBRC11486
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Gm1673<em1Osb> Tg(CAG-Gm1673/PA/1D4)1Osb
Former Common nameGm1673<-1862/wt>, CAG-Gm1673-PA/1D4 Tg #1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Mixed genetic background of C57BL/6 x DBA/2.
Strain descriptionGm1673 KO mouse generated by the CRISPR/Cas9. 1862 bp deletion in the Gm1673 gene. This strain also has atransgene expressing Gm1673 fused with PA-1D4-tag (C-terminus) under the control of CAG promoter.
Colony maintenance
ReferencesNat. Commun., 14(1):2354 (2023). doi: 10.1038/s41467-023-37984-x. 37095084

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gm1673predicted gene, 16735Gm1673<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
1D41D4 tag (synthetic)UN1D4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gm1673predicted gene 1673UNGm1673CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PAPA tag (GVAMPGAEDDVV) (synthetic) human PodoplaninUNPA

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Gm1673 gene, chicken beta-actin promoter, CMV CMV IE enhancer, mouse Gm1673 cDNA, synthetic PA tag and 1D4 tag, rabbit globin polyA signal
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 14(1):2354 (2023). doi: 10.1038/s41467-023-37984-x.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data