Strain Data Sheet
RBRC11486
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11486 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6D2-Gm1673<em1Osb> Tg(CAG-Gm1673/PA/1D4)1Osb |
| Former Common name | Gm1673<-1862/wt>, CAG-Gm1673-PA/1D4 Tg #1 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Mixed genetic background of C57BL/6 x DBA/2. |
| Strain description | Gm1673 KO mouse generated by the CRISPR/Cas9. 1862 bp deletion in the Gm1673 gene. This strain also has atransgene expressing Gm1673 fused with PA-1D4-tag (C-terminus) under the control of CAG promoter. |
| Colony maintenance | |
| References | A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility. Daiji Kiyozumi, Kentaro Shimada, Michael Chalick, Chihiro Emori, Mayo Kodani, Seiya Oura, Taichi Noda, Tsutomu Endo, Martin M Matzuk, Daniel H Wreschner, Masahito Ikawa Nat. Commun., 14(1):2354 (2023). 37095084 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Gm1673 MGI:2686519 | predicted gene, 1673 | 5 | Gm1673<em1Osb> | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
| 1D4 | 1D4 tag (synthetic) | UN | 1D4 | ||||
| Gm1673 | predicted gene 1673 | UN | Gm1673 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| PA | PA tag (GVAMPGAEDDVV) (synthetic) human Podoplanin | UN | PA | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Gm1673 gene, chicken beta-actin promoter, CMV CMV IE enhancer, mouse Gm1673 cDNA, synthetic PA tag and 1D4 tag, rabbit globin polyA signal |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 14(1):2354 (2023). doi: 10.1038/s41467-023-37984-x.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |