Strain Data Sheet

RBRC11458

Strain Information

Image
BRC No.RBRC11458
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Clgn-Nell2/His)1Osb
Former Common nameClgn-Nell2-His Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionTransgenic mice expressing Nell2/6xHis tag fusion protein under the control of Clgn promoter.
Colony maintenance
References
NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility.
Kiyozumi D, Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren K M, Horie M, Okabe M, Matzuk M M, Ikawa M
Science, 368(6495):1132-1135 (2020). 32499443

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
His His tag (synthetic)UNHis
Nell2 NEL-like 2UNNell2 mouse Clgn promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAMouse calmegin promoter, Rabbit beta-globin polyadenylation signal, Mouse Nell2 cDNA, synthetic His
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 368(6495):1132-1135 (2020). doi: 10.1126/science.aay5134.
Science. Jun 5;368(6495):1132-1135.(2020) doi: 10.1126/science.aay5134.

RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data