Strain Information | |
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Image | |
BRC No. | RBRC11438 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#16Ysa |
Former Common name | pMK411-#16 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Yumiko Saga, National Institute of Genetics in 2021. This strain was generated using fertilized eggs derived from BDF1, then backcrossed to C57BL/6J twice. |
Strain description | Transgenic mice (Line 16) expressing EGFP reporter and nanoluc fused with OsTIR and AID. |
Colony maintenance | |
References | Nat. Commun., 11(1):5701 (2020). doi: 10.1038/s41467-020-19532-z. 33177522 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/09Room:3-ARack:I 2023/10/10Room:3-ARack:I |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter NanoLucluciferase (Oplophorus gracilirostris)UNNanoLuc Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptideUNP2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter TIR1F-box transport inhibitor response 1 (Oryza sativa)UNTIR1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mAIDmAID (Arabidopsis thaliana)UNmAID |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Oryza sativa TIRI(F74G) cDNA, Porcie teschovirus P2A, Arabidopsis thaliana mAID cDNA, Aequorea victoria EGFP cDNA, Oplophorus gracilirostris luciferase cDNA, SV40 pA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 11(1):5701 (2020). doi: 10.1038/s41467-020-19532-z.The RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Yumiko Saga (National Institute of Genetics) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |