Strain Data Sheet
RBRC11432
Strain Information | |
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| Image | |
| BRC No. | RBRC11432 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Flt1<tm2Msh> |
| Former Common name | FLT1<TM-TK-/- > mice, FLT1<TM-TK+/-> mice |
| H-2 Haplotype | |
| ES Cell line | CCE/EK.CCE [129S/SvEv-Gpi1<c>] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masabumi Shibuya, Jobu University in 2021. This strain was generated using CCE/EK.CCE (ES cell) derived from 129S/SvEv-Gpi1<c>. Mice were backcrossed to C57BL.6. Mixed genetic background. |
| Strain description | Flt1 mutant mice lacking kinase and transmembrane domain. |
| Colony maintenance | |
| References | Membrane fixation of vascular endothelial growth factor receptor 1 ligand-binding domain is important for vasculogenesis and angiogenesis in mice. Hiratsuka S, Nakao K, Nakamura K, Katsuki M, Maru Y, Shibuya M Mol Cell Biol., 25(1):346-54 (2005). 15601855 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Flt1 MGI:95558 | FMS-like tyrosine kinase 1 | 5 | Flt1<tm2Msh> MGI:3527129 | targeted mutation 2, Masabumi Shibuya | |||
| neo | neomycin resistance gene (E. coli) | 5 | |||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | E. coli Neomycin-resistant gene, Mouse FLT1 gene: Intron-15, -16の一部、Exon-17とその下流（参考文献１、Figure 1参照） |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol Cell Biol., 25, 346-354 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Masabumi Shibuya (Jobu University) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |