Strain Data Sheet

RBRC11412

Strain Information

Image
BRC No.RBRC11412
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet1<tm1.1Hko>
Former Common nameTET1exon2
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeposited by Haruhiko Koseki, RIKEN IMS in 2021. This strain was generated using M1 ES cells derived from (C57BL/6 x 129)F1 and mice were backcrossed to C57BL/6 several times. A Frt flanked neo cassette was removed from this strains.
Strain descriptionTet1 floxed mice. A pair of loxP flanks exon 2 of the Tet1 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/02/26Room:4-CRack:D
2023/11/27Room:4-CRack:D
2023/08/28Room:4-CRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tet1tet methylcytosine dioxygenase 110Tet1<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Tet1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or for-profit use based on the results from the use of the
BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN IMS)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data