Strain Information | |
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Image | |
BRC No. | RBRC11404 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6.Cg-Herc3<em1Tfur> |
Former Common name | Herc3 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Takahisa Furukawa, Osaka University in 2021. This strain was generated using fertilized eggs derived from B6C3F1 and mice were further backcrossed to C57BL/6J for six generations. |
Strain description | Herc3 mutant mice lacking 38 bp deletion at exon 2 which results in premature translation termination. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
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Donor DNA | Streptococcus pyogenes U6 promoter, Streptococcus pyogenes gRNA scaffold, human U6 terminator, CMV, chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal (NLS)Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal,AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka University. |
Depositor | Takahisa Furukawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |