Strain Data Sheet

RBRC11401

Strain Information

Image
BRC No.RBRC11401
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Cdkn1a<em1Matsu> Cdkn2a<em1Matsu>
Former Common nameP16/19<+/->; p21<-/-> DKO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Makoto Matsuyama, Shigei Medical Research Institute in 2021. C57BL/6H genetic background.
Strain descriptionDouble mutant mice for Cdkn1a and Cdkn2a. This strain was generated by the CRISPR/Cas9. Stop codon was inserted at exon 2 and exon 3 of the Cdkn1a and Cdkn2a, respectively.
Colony maintenance
ReferencesSci. Rep., 19;9(1):12059 (2019). 31427681

Health Report

Examination Date / Room / Rack2023/02/27Room:4-BRack:B
2022/11/29Room:4-BRack:B
2022/08/29Room:4-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdkn1acyclin-dependent kinase inhibitor 1A (P21)17Cdkn1a<em1Matsu>endonuclease-mediated mutation 1, Makoto Matsuyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdkn2acyclin dependent kinase inhibitor 2A4Cdkn2a<em1Matsu>endonuclease-mediated mutation 1, Makoto Matsuyama

Ordering Information

Donor DNA
Research applicationCell Biology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2019 Aug 19;9(1):12059.
DepositorMakoto Matsuyama (Shigei Medical Research Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data