Strain Data Sheet

RBRC11397

Strain Information

Image
BRC No.RBRC11397
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.129P2(B6)-Il5ra<tm1Ktk>
Former Common nameBALB/c IL-5R alpha -/-
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Kiyoshi Takatsu, Institute of Medical Science in 1996. E14.1 ES cells were used. The mutant mice were backcrossed to BALB/c.
Strain descriptionIL-5Ralpha gene knockout mice. A translational stop codon was introduced into exon 5 of the Il5ra and the exon 6 was replaced with a neo cassette.
Colony maintenance
ReferencesImmunity, 4(5):483-94 (1996). 8630733
Immunology, 102(3):289-300 (2001). 11298827

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il5rainterleukin 5 receptor, alpha6Il5ra<tm1Ktk>targeted mutation 1, Kiyoshi Takatsu

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAMouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Mouse IL-5R alpha genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity 4, 483-494 (1996)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in the articles when the users publish their data using these mice for 1 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice.
DepositorKiyoshi Takatsu (Toyama Prefectural Institute for Pharmaceutical Research)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data