Strain Data Sheet

RBRC11395

Strain Information

Image
BRC No.RBRC11395
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S6/SvEv-Samd7<tm1Tfur>
Former Common nameSamd7 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takahisa Furukawa, Osaka University in 2021. This strain was generated using TC1/TC-1 (ES Cell) derived from 129S6/SvEvTac. 129S6 genetic background.
Strain descriptionKncokout mice for Samd7. Exon 4 to 6 of the Samd7 gene were deleted.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 114(39):E8246-E8273 (2017). 28893993

Health Report

Examination Date / Room / Rack2023/02/20Room:4-ARack:A
2022/11/21Room:4-ARack:A
2022/08/22Room:4-ARack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Samd7sterile alpha motif domain containing 73Samd7<tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, mouse Samd7 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA. 2017 Sep 26;114(39):E8246-E8273Prior to the requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data