Strain Data Sheet

RBRC11383

Strain Information

Image
BRC No.RBRC11383
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Mir124a-1hg<tm1Tfur>
Former Common nameRncr3<-/->, miR-124-1<-/->
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeposited by Takahisa Furukawa, Osaka University in 2021. This strain was generated using TC1/TC-1 (ES Cell) derived from 129S6/SvEvTac. Mice were further backcrossed to C57BL/6N for 5 generations.
Strain descriptionKnockout mice for miR-124a-1.
Colony maintenance
ReferencesNat. Neurosci., 14(9) 1125-34 (2011). doi: 10.1038/nn.2897. 21857657
Sci. Rep., 9(1) 3445 (2019). doi:10.1038/s41598-019-38910-2. 30837489

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mir124a-1hgMir124-1 host gene (non-protein coding)14Mir124a-1hg<tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal14

Ordering Information

Donor DNAMouse PGK promoter, E. coli Neomycin resistance gene, SV40 PolyA signal, Mouse Rncr3 locus
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Neuronscience vol. 14,9 1125-34. 21 Aug. 2011,doi: 10.1038/nn.2897
Scientific reports vol. 9,1 3445. 5 Mar. 2019, doi:10.1038/s41598-019-38910-2In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to the requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data