Strain Data Sheet

RBRC11380

Strain Information

Image
BRC No.RBRC11380
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-F8<em2Tsuka>
Former Common nameF8 knockout mice(CRISPR/Cas9)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Tsukasa Ohmori, Jichi Medical University in 2021. C57BL/6J genetic background.
Strain descriptionF8 mutant mice generated by the CRISPR/Cas9. 7 bp deletion at exon 14 of the F8 gene. Mutant mice have only 2% of factor VIII activity and tend to bleeding.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F8coagulation factor VIIIXF8<em2Tsuka>endonuclease-mediated mutation 2, Tsukasa Ohmori

Ordering Information

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTsukasa Ohmori (Jichi Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data