Strain Data Sheet

RBRC11377

Strain Information

Image
BRC No.RBRC11377
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Atf5<em1(Atf5/HA)Yuta>
Former Common nameATF5-HA knock-in, B6-ATF5HA KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Haruo Nakano, Tokyo university of the pharmacy and life sciences in 2019.The HA-tag was introduced into zygotes by CRISPR/Cas9 genome editing. C57BL/6NJcl (CLEA Japan Inc.)The ATF5-HA knock-in male G0 offspring was mated with wild type females, and then propageted by sibling crossing (Heterozygous x Heterozygous or Homozygous x Homozygous).
Strain descriptionATF5-Hemagglutinin (HA) knock-in mice. A HA-tag sequence was integrated into the stop codon site of ATF5 gene at an in-flame position.
Colony maintenance
ReferencesCell Tissue Res., (2021). doi: 10.1007/s00441-021-03450-7. 33825962

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Atf5activating transcription factor 57Atf5<em1(Atf5/HA)Yuta>endonuclease-mediated mutation 1, Yuji Takahashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence7HA

Ordering Information

供与核酸influenza virus HA (hemagglutinin) tag sequence
Research application
提供条件条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。
研究成果の公表にあたって寄託者の指定する文献を引用する。Cell Tissue Res. 2021 Apr 7. doi: 10.1007/s00441-021-03450-7.
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor高橋　勇二（東京薬科大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data