Strain Information | |
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Image | |
BRC No. | RBRC11375 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6.B6D2-Hmces<em1Nsas> |
Former Common name | B6-Hmces<em1> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Nobuya Sasaki, Kitasato University in 2021. This strain was generated using fertilized eggs derived from BDF1. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic strain. |
Strain description | Hmces mutants generated by the CRISPR/Cas9. 1 bp insertion at exon 2 of the Hmces gene. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack | 2023/02/27Room:4-BRack:I 2022/11/29Room:4-BRack:I 2022/08/29Room:4-BRack:I 2022/05/31Room:4-BRack:I |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hmces5-hydroxymethylcytosine (hmC) binding, ES cell specific6Hmces<em1Nsas>endonuclease-mediated mutation 1, Nobuya Sasaki |
Ordering Information | |
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Donor DNA | S. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene mRNA, Mouse Hmces gene RNA flagment (Single quide RNA)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | Metabolism Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration. |
Depositor | Nobuya Sasaki (Kitasato University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |