Strain Data Sheet

RBRC11331

Strain Information

Image
BRC No.RBRC11331
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6.Cg-Rpe65<em1Tfur>
Former Common nameRpe65 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takahisa Furukawa, Osaka University in 2021. Mice were generated using fertilized eggs derived from BDF1. Mice were backcrossed to C57BL/6J for 4 generations.
Strain descriptionRpe65 mutant mice. 4 bp deletion at exon 2.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rpe65retinal pigment epithelium 653Rpe65<em1Tfur>endonuclease-mediated mutation 1, Takahisa Furukawa

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9 [Streptococcus pyogenes U6 promoter, Streptococcus pyogenes gRNA scaffold, human U6 terminator, CMV, chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS)Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data