Strain Information | |
---|---|
Image | |
BRC No. | RBRC11331 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6.Cg-Rpe65<em1Tfur> |
Former Common name | Rpe65 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Takahisa Furukawa, Osaka University in 2021. Mice were generated using fertilized eggs derived from BDF1. Mice were backcrossed to C57BL/6J for 4 generations. |
Strain description | Rpe65 mutant mice. 4 bp deletion at exon 2. |
Colony maintenance | |
References |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Rpe65retinal pigment epithelium 653Rpe65<em1Tfur>endonuclease-mediated mutation 1, Takahisa Furukawa |
Ordering Information | |
---|---|
Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9 [Streptococcus pyogenes U6 promoter, Streptococcus pyogenes gRNA scaffold, human U6 terminator, CMV, chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS)Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka University. |
Depositor | Takahisa Furukawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
---|
No Data |