Strain Data Sheet

RBRC11325

Strain Information

Image
BRC No.RBRC11325
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Prkdc<em2(R2140C)Nsas>
Former Common nameB6-Prkdc<R2140C>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Nobuya Sasaki, Kitasato University in 2021. C57BL/6J genetic background.
Strain descriptionPrkdc mutants harboring a point mutation (R2140C) generated by the CRISPR/Cas9.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<em2(R2140C)Nsas>endonuclease-mediated mutation 2, Nobuya Sasaki

Ordering Information

Donor DNAS. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene mRNA, Mouse Prkdc gene RNA flagment (Single quide RNA), Mouse Mutant Prkdc gene single-strand ODN* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data