Strain Information | |
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Image | |
BRC No. | RBRC11325 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6-Prkdc<em2(R2140C)Nsas> |
Former Common name | B6-Prkdc<R2140C> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Deposited by Nobuya Sasaki, Kitasato University in 2021. C57BL/6J genetic background. |
Strain description | Prkdc mutants harboring a point mutation (R2140C) generated by the CRISPR/Cas9. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Prkdcprotein kinase, DNA activated, catalytic polypeptide16Prkdc<em2(R2140C)Nsas>endonuclease-mediated mutation 2, Nobuya Sasaki |
Ordering Information | |
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Donor DNA | S. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene mRNA, Mouse Prkdc gene RNA flagment (Single quide RNA), Mouse Mutant Prkdc gene single-strand ODN* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration. |
Depositor | Nobuya Sasaki (Kitasato University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |