Strain Data Sheet

RBRC11317

Information1

Image
BRC No.RBRC11317
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6.B6D2-Cma1<em1(HBEGF)Nsas>
Former Common nameMcpt5/Cma1<DTR>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nobuya Sasaki, Kitasato University in 2021. This strain was generated using fertilized eggs derived from BDF1. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic strain.
Strain descriptionHBEGF knockin mice at the translation initiation codon of the Cma1 gene.
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 551:127-132 (2021). 33725574

Health Report

Examination Date / Room / Rack2023/02/20Room:4-ARack:L
2022/12/05Room:4-ARack:L
2022/11/21Room:4-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cma1chymase 1, mast cell14Cma1<em1(HBEGF)Nsas>endonuclease-mediated mutation 1, Nobuya Sasaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)14HBEGF

Information2

Donor DNAS. pyogenes crRNA and tracrRNA, Mouse Cma1 gene RNA flagment (crRNA), Mouse Cma1 genome DNA, Human HBEGF (DTR) coding sequence DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data