Strain Data Sheet

RBRC11315

Strain Information

Image
BRC No.RBRC11315
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cadps2<tm1.1Ksak>
Former Common nameCadps2-flox knock-in mice
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Teiichi Furuichi, Tokyo University of Science in 2021. Mice were generated using RENKA ES cells. C57BL/6 genetic background
Strain descriptionCadps2 floxed mice. A pair of loxP flanks exon 6.
Colony maintenance
ReferencesJ. Neurosci., 41(20):4524-4535 (2021). 33846232
J. Clin. lnvest., 117, 931-943 (2007) . 17380209
J. Neurosci., 27, 2472-2482 (2007). 17344385

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cadps2Ca2+-dependent activator protein for secretion 26Cadps2<tm1.1Ksak>targeted mutation 1.1, Kenji Sakimur

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Ordering Information

Donor DNAP1 Phage (class 1) loxP sites (M10287), Saccharomyces cerevisiae (class 1) FRT site(J01347.1), Mus musculus CAPS2/Cadps2 genomic DNA(NM_153163)
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Neurosci. 2021 May 19;41(20):4524-4535. doi: 10.1523/JNEUROSCI.3240-20.2021.Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Kenji Sakimura; sakimura@bri.niigata-u.ac.jp) using the Approval Form.
DepositorTeiichi furuichi (Tokyo University of Science)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data