Strain Data Sheet

RBRC11304

Strain Information

Image
BRC No.RBRC11304
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6J.B6N-Chdh<em1Rmps>
Former Common nameChdh KO mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takeo Yoshikawa, RIKEN in 2021. This strain was generated by the CRISPR/Cas9. Mice were further backcrossed to C57BL/6J. C57BL/6J congenic line.
Strain descriptionChdh mutant mice deleting 43 bp shortly after translation initiation codon.
Colony maintenance
ReferencesEBioMedicine. 45, 432-446 (2019). 31255657

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Chdhcholine dehydrogenase14Chdh<em1Rmps>endonuclease-mediated mutation 1, Takeo Yoshikawa

Ordering Information

Donor DNAStreptococcus Pyogenes Cas9 nickase
Research applicationMetabolism Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine. 45, 432-446 (2019).
DepositorTakeo Yoshikawa (RIKEN CBS)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data