Strain Data Sheet

RBRC11302

Strain Information

Image
BRC No.RBRC11302
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(CAG-Mpst)498Rmps
Former Common nameMpst-Tg mouse (Line 498)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takeo Yoshikawa, RIKEN in 2021. C57BL/6N genetic background.
Strain descriptionTransgenic mice expressing HA-tagged mouse Mpst(3-mercaptopyruvate sulfurtransferase) under the control of the CAG promoter. Line 498.
Colony maintenance
ReferencesEMBO Mol. Med., 11:e10695 (2020). 31657521

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
AmpAmpicillin resistance gene (E. coli)UNAmp

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequenceUNHA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyAUNHBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mpstmercaptopyruvate sulfurtransferaseUNMpstCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), human influenza virus HA (hemagglutinin) tag sequence, mouse Mpst cDNA, SV40 polyA signal, E. coli pBR322 Replication origin, E. coli Ampicillin resistance gene
Research applicationMouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO Mol. Med., 11:e10695 (2020).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorTakeo Yoshikawa (RIKEN CBS)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data